R-loop是一种由RNA：DNA杂合链和单链DNA组成的特殊核酸结构，在原核和真核生物的基因组中分布广泛且普遍存在。R-loop在很多关键的生物学过程中发挥重要功能，包括染色质修饰、转录调控、DNA损伤修复以及基因组稳定性等，但其如何被精确调控的机制尚不清楚。

本研究针对现有R-loop预测方法仅依赖DNA序列特性、无法捕捉细胞类型特异性的局限，开发了整合高通量测序特征与序列信息的机器学习模型。通过分析人类HEK293、K562和HeLa细胞系数据，发现转录组（RNA-seq）、染色质可及性（DNase-seq）和组蛋白标记（H3K36me3）最具 ...

本研究聚焦R-Loop（DNA:RNA杂交结构）在癌症治疗耐药性中的作用，揭示了其通过干扰基因组稳定性、表观遗传调控及DNA损伤修复通路促进肿瘤进展的机制。团队系统分析了经典R-Loop调控因子（如RNase H、SETX等）的功能缺失如何诱发基因组不稳定，并筛选出靶向诱导R ...

This new review article highlights the pivotal and paradoxical role of R-loops in maintaining genomic stability while simultaneously posing risks to it. These three-stranded nucleic acid structures, ...

Genetically speaking, it’s a bacterium’s worst-case scenario: during transcription, newly minted RNA sticks to its DNA template, forming a 3-stranded structure known as an R-loop. While these ...

Image Caption: The factors contributing to R-loop formation. Several factors contribute to R-loop formation, including RNA components, specific DNA structures, histone and RNA modifications, ...

A new study by researchers at the German Center for Infection Research (DZIF) and Heidelberg University Hospital has identified RNA:DNA hybrids, known as R-loops, as structural features that guide the ...

“There are currently limited therapeutic options for elderly patients with myeloid malignancies, who are ineligible for stem cell transplantation,” commented Dr. Sayantani Sinha, a postdoctoral ...